pTwist-Lenti-CMV-BSD-PC-WT-3xFLAG
(Plasmid
#258083)
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PurposeOverexpression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 258083 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepTwist-Lenti-CMV-BSD
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Backbone manufacturerTwist Bioscience
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Vector typeLentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePC
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Alt namePCB
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Alt namePYC
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SpeciesH. sapiens (human)
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Insert Size (bp)3629
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Entrez GenePC (a.k.a. PCB)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer WPRE-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTwist-Lenti-CMV-BSD-PC-WT-3xFLAG was a gift from Alexis Jourdain (Addgene plasmid # 258083 ; http://n2t.net/addgene:258083 ; RRID:Addgene_258083)