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IVT-CHARM-
(Plasmid #258407)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258407 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pbluescript
  • Vector type
    CRISPR ; IVT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CHARM (without KRAB)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.03.27.714536 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IVT-CHARM- was a gift from Vijay Sankaran (Addgene plasmid # 258407 ; http://n2t.net/addgene:258407 ; RRID:Addgene_258407)

  • For your References section:

    Epigenome editing of human hematopoietic stem cells enables sustained and reversible thrombosis prevention. Ye T, Xu W, Barrachina MN, Lyu P, Antoszewski M, della Volpe L, Guo C, Lee AJ, Theardy MS, Shelton SD, Wahlster L, Caulier A, Messa L, Poeschla M, Agarwal G, Mitra R, Schmaier AA, Weissman JS, Machlus KR, Sankaran VG. bioRxiv 2026.03.27.714536; doi: 10.64898/2026.03.27.714536 10.64898/2026.03.27.714536
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