Skip to main content
Addgene
Open Menu

p300_N1196D_R1234E_GFP
(Plasmid #258517)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258517 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pIRESpuro2
  • Total vector size (bp) -2
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FRB-EGFP-p300 core
  • Alt name
    p300
  • Alt name
    KAT3B
  • Alt name
    MKHK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2907
  • GenBank ID
    NM_001429
  • Entrez Gene
    EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
  • Promoter EF1alpha
  • Tags / Fusion Proteins
    • EGFP (N-terminal of p300 core) (N terminal on insert)
    • FRB (N-terminal of EGFP) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAAGTGCAGTAGTCGCCGTGAACGT
  • 3′ sequencing primer CATGCCCGCTTTTGAGA
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mutations were introduced in plasmid eGFP-p300 Addgene plasmids #191764 from Daniel Gerlich.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutations were introduced in plasmid eGFP-p300 Addgene plasmids #191764 from Daniel Gerlich.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p300_N1196D_R1234E_GFP was a gift from Geert van den Bogaart (Addgene plasmid # 258517 ; http://n2t.net/addgene:258517 ; RRID:Addgene_258517)

  • For your References section:

    Butyrate causes loss of specific acetylation by the histone acetyltransferase p300. Jiang M, Psoma A, Lyu J, Chiariello MG, Modderman R, Bianchi F, Incarnato D, Marrink SJ, Jonkers IH, van den Bogaart G. iScience. 2026 Jul 8;29(7):116528. doi: 10.1016/j.isci.2026.116528. eCollection 2026 Jul 17. 10.1016/j.isci.2026.116528 PubMed 42491727
Related items: