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phage UbiC ALFA-Nanobody-sfGFP-GB1
(Plasmid #258550)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258550 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pHAGE-UbiC
  • Backbone size w/o insert (bp) 5165
  • Total vector size (bp) 9596
  • Vector type
    Mammalian Expression, Lentiviral, Affinity Reagent/ Antibody

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-ALFA nanobody fused with sfGFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    369
  • Promoter UbiC
  • Tags / Fusion Proteins
    • GB1 (C terminal on insert)
    • sfGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.29.685314 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phage UbiC ALFA-Nanobody-sfGFP-GB1 was a gift from Jeffrey Chao (Addgene plasmid # 258550 ; http://n2t.net/addgene:258550 ; RRID:Addgene_258550)

  • For your References section:

    Quantitative comparison of methodologies for translation site imaging in living cells. Misiaszek AD, Griesbach E, Jaugaite E, Ortale A, Eglinger J, Hochstoeger T, Chao JA. RNA. 2026 Jun 16:rna.080881.125. doi: 10.1261/rna.080881.125. 10.1261/rna.080881.125 PubMed 42303464
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