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Quantitative comparison of methodologies for translation site imaging in living cells.

Misiaszek AD, Griesbach E, Jaugaite E, Ortale A, Eglinger J, Hochstoeger T, Chao JA
RNA. 2026 Jun 16:rna.080881.125. doi: 10.1261/rna.080881.125. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
258544EGFP-Renilla-FKBP12-24xMS2mRNA reporter construct with EGFP-Renilla-FKBP12-24xMS2 under Tet-On promoter
258545sm-18xGCN4-Renilla-FKBP12-24xMS2mRNA reporter construct with 18xGCN4-spaghetti monster (sm)-Renilla-FKBP12-24xMS2 under Tet-On promoter
258546sm-18xHA-Renilla-FKBP12-24xMS2mRNA reporter construct with 18xHA-spaghetti monster (sm)-Renilla-FKBP12-24xMS2 under Tet-On promoter
258547sm-18xALFA-Renilla-FKBP12-24xMS2mRNA reporter construct with 18xALFA-spaghetti monster (sm)-Renilla-FKBP12-24xMS2 under Tet-On promoter
25854818xGCN4-Renilla-FKBP12-24xMS2mRNA reporter construct with 18xGCN4 (linear)-Renilla-FKBP12-24xMS2 under Tet-On promoter
258549phage UbiC HA-Frankenbody-sfGFP-GB1HA-Frankenbody-sfGFP-GB1 for lentivirus integration
258550phage UbiC ALFA-Nanobody-sfGFP-GB1ALFA-Nanobody-sfGFP-GB1 for lentivirus integration
258551phage UbiC HA-Frankenbody-GB1-HaloHA-Frankenbody-GB1-Halo for lentivirus integration

Antibodies from Article