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sm-18xGCN4-Renilla-FKBP12-24xMS2
(Plasmid #258545)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258545 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSF4
  • Backbone size w/o insert (bp) 5729
  • Total vector size (bp) 11601
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla luciferase
  • Species
    Renilla reniformis
  • Insert Size (bp)
    930
  • Promoter Tet-ON
  • Tags / Fusion Proteins
    • 18xGCN4 spaghetti monster (N terminal on insert)
    • FKBP (C terminal on insert)
    • 24xMS2v5 stem loops in 3'UTR
    • CTE in 3'UTR

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.29.685314 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sm-18xGCN4-Renilla-FKBP12-24xMS2 was a gift from Jeffrey Chao (Addgene plasmid # 258545 ; http://n2t.net/addgene:258545 ; RRID:Addgene_258545)

  • For your References section:

    Quantitative comparison of methodologies for translation site imaging in living cells. Misiaszek AD, Griesbach E, Jaugaite E, Ortale A, Eglinger J, Hochstoeger T, Chao JA. RNA. 2026 Jun 16:rna.080881.125. doi: 10.1261/rna.080881.125. 10.1261/rna.080881.125 PubMed 42303464
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