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pTRIPZ-EC-hBRD2shRNA(KH320)
(Plasmid #258560)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258560 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTRIPZ-EC
  • Backbone manufacturer
    Hu Lab
  • Backbone size w/o insert (bp) 13303
  • Total vector size (bp) 13404
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human bromodomain extra terminal protein 2
  • Alt name
    BRD2
  • gRNA/shRNA sequence
    CCCGGAAGCCCTACACCATTA
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_005104
  • Entrez Gene
    BRD2 (a.k.a. BRD2-IT1, D6S113E, FSH, FSHRG1, FSRG1, NAT, O27.1.1, RING3, RNF3)
  • Promoter CMV minimum with TRE repeat

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer RFP-F, p653: tggctgtggccaagtactgc
  • 3′ sequencing primer TRIP-MLU, p634: ggccacgcgtcctaggtaa
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIPZ-EC-hBRD2shRNA(KH320) was a gift from Kejin Hu (Addgene plasmid # 258560 ; http://n2t.net/addgene:258560 ; RRID:Addgene_258560)

  • For your References section:

    BRD2 impedes iPSC reprogramming by regulating lipid biosynthesis and the matrisome via its ET tail. Cevallos RR, Zhang R, Sian R, Chen SG, Hu K. Commun Biol (2026). doi: 10.1038/s42003-026-10691-1 10.1038/s42003-026-10691-1
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