pET-LIC-cloning vector (2A-T) No Golden Gate sites BsaI and Esp3I
(Plasmid
#260643)
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Purpose(Empty Backbone) LIC cloning vector with Golden Gate sites BsaI and Esp3I removed
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260643 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET-LIC-cloning vector (2A-T)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-LIC-cloning vector (2A-T) No Golden Gate sites BsaI and Esp3I was a gift from Stan Fields (Addgene plasmid # 260643 ; http://n2t.net/addgene:260643 ; RRID:Addgene_260643) -
For your References section:
Dosa: A method to covalently barcode proteins for high-throughput biochemistry. Ashok Y, Bubb KL, Oy C, Gorjifard S, Cuperus JT, Queitsch C, Fields S. Proc Natl Acad Sci U S A. 2026 Jan 6;123(1):e2529762123. doi: 10.1073/pnas.2529762123. Epub 2026 Jan 2. 10.1073/pnas.2529762123 PubMed 41481464