Dosa: A method to covalently barcode proteins for high-throughput biochemistry.
Ashok Y, Bubb KL, Oy C, Gorjifard S, Cuperus JT, Queitsch C, Fields S
Proc Natl Acad Sci U S A. 2026 Jan 6;123(1):e2529762123. doi: 10.1073/pnas.2529762123. Epub 2026 Jan 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 260641 | E. coli TrmA (E358Q) | Expression of tRNA Crosslinking mutant TrmA (E358Q) in bacteria |
| 260642 | E.coli TrmA (E358Q, C324A) | Expression of dead mutant TrmA (E358Q, C324A) in bacteria |
| 260643 | pET-LIC-cloning vector (2A-T) No Golden Gate sites BsaI and Esp3I | LIC cloning vector with Golden Gate sites BsaI and Esp3I removed |
| 260647 | pDosa1 | Cloning vector to express a fusion of a variant protein and a tRNA modifying enzyme TrmA (E358Q) to covalently link an RNA barcode |
| 260649 | GM6 Aβ(1-42)-pDosa1 | Bacterial expression of GM6 mutant amyloid beta (1-42) fused to tRNA modifying enzyme TrmA (E358Q) |
| 260650 | WT MBP-pDosa1 | Bacterial expression of MBP fused to tRNA modifying enzyme TrmA (E358Q) |
| 260651 | MBP Y283D-pDosa1 | Bacterial expression of mutated MBP fused to tRNA modifying enzyme TrmA (E358Q) |
| 260652 | MBP G32D, I33P-pDosa1 | Bacterial expression of mutated MBP fused to tRNA modifying enzyme TrmA (E358Q) |