E.coli TrmA (E358Q, C324A)
(Plasmid
#260642)
-
PurposeExpression of dead mutant TrmA (E358Q, C324A) in bacteria
-
Depositing Lab
-
Depositing OrganizationUniversity of Washington
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260642 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepNIC28-Bsa4
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameTrmA
-
SpeciesE. coli
- Promoter T7
-
Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
E.coli TrmA (E358Q, C324A) was a gift from Stan Fields (Addgene plasmid # 260642 ; http://n2t.net/addgene:260642 ; RRID:Addgene_260642) -
For your References section:
Dosa: A method to covalently barcode proteins for high-throughput biochemistry. Ashok Y, Bubb KL, Oy C, Gorjifard S, Cuperus JT, Queitsch C, Fields S. Proc Natl Acad Sci U S A. 2026 Jan 6;123(1):e2529762123. doi: 10.1073/pnas.2529762123. Epub 2026 Jan 2. 10.1073/pnas.2529762123 PubMed 41481464