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WT MBP-pDosa1
(Plasmid #260650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 260650 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pDosa1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MBP
  • Species
    E. coli
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • TrmA (E358Q) (C terminal on backbone)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WT MBP-pDosa1 was a gift from Stan Fields (Addgene plasmid # 260650 ; http://n2t.net/addgene:260650 ; RRID:Addgene_260650)

  • For your References section:

    Dosa: A method to covalently barcode proteins for high-throughput biochemistry. Ashok Y, Bubb KL, Oy C, Gorjifard S, Cuperus JT, Queitsch C, Fields S. Proc Natl Acad Sci U S A. 2026 Jan 6;123(1):e2529762123. doi: 10.1073/pnas.2529762123. Epub 2026 Jan 2. 10.1073/pnas.2529762123 PubMed 41481464
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