pDosa1
(Plasmid
#260647)
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Purpose(Empty Backbone) Cloning vector to express a fusion of a variant protein and a tRNA modifying enzyme TrmA (E358Q) to covalently link an RNA barcode
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Depositing Lab
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Depositing OrganizationUniversity of Washington
Located in the United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET-LIC-cloning vector (2A-T)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Protein of interest to be cloned into BsaI site. Barcodes to be inserted into Esp3I site. Custom primers for NGS sequencing. Fig S14A.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDosa1 was a gift from Stan Fields (Addgene plasmid # 260647 ; http://n2t.net/addgene:260647 ; RRID:Addgene_260647) -
For your References section:
Dosa: A method to covalently barcode proteins for high-throughput biochemistry. Ashok Y, Bubb KL, Oy C, Gorjifard S, Cuperus JT, Queitsch C, Fields S. Proc Natl Acad Sci U S A. 2026 Jan 6;123(1):e2529762123. doi: 10.1073/pnas.2529762123. Epub 2026 Jan 2. 10.1073/pnas.2529762123 PubMed 41481464
