pET26b S5a(196-306)
(Plasmid
#260881)
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PurposeS5a(196-306) contains two ubiquitin-interacting motifs (UIMs) through which S5a/RPN10/PSMD4 recruits ubiquitylated substrates to the proteasome for their degradation.
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Depositing Lab
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Depositing OrganizationUniversity of Minnesota
Located in the United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET-26b(+)
- Total vector size (bp) 5360
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePSMD4 (196-306)
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SpeciesH. sapiens (human)
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Insert Size (bp)393
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GenBank IDNM_002810 NM_002810
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Tag
/ Fusion Protein
- 10xHis-tag (N terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET26b S5a(196-306) was a gift from Kylie Walters (Addgene plasmid # 260881 ; http://n2t.net/addgene:260881 ; RRID:Addgene_260881) -
For your References section:
Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition. Wang Q, Young P, Walters KJ. J Mol Biol. 2005 May 6;348(3):727-39. doi: 10.1016/j.jmb.2005.03.007. 10.1016/j.jmb.2005.03.007 PubMed 15826667
