pGEX-6P S5a (196-272)
(Plasmid
#260882)
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PurposeS5a/RPN10/PSMD4 ubiquitin-interacting motif 1 (UIM1)
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Depositing Lab
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Depositing OrganizationUniversity of Minnesota
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260882 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepGEX6P1
- Backbone size w/o insert (bp) 4984
- Total vector size (bp) 5212
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePSMD4 (196-272)
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SpeciesH. sapiens (human)
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Insert Size (bp)234
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GenBank IDNM_002810 NM_002810
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Tag
/ Fusion Protein
- GST-tag (N terminal on backbone)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6P S5a (196-272) was a gift from Kylie Walters (Addgene plasmid # 260882 ; http://n2t.net/addgene:260882 ; RRID:Addgene_260882) -
For your References section:
Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition. Wang Q, Young P, Walters KJ. J Mol Biol. 2005 May 6;348(3):727-39. doi: 10.1016/j.jmb.2005.03.007. 10.1016/j.jmb.2005.03.007 PubMed 15826667