pRSET hRpn13 (1-150)
(Plasmid
#260902)
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PurposehRpn13 Pru (1–150) was expressed in E. coli BL21(DE3) pLysS cells as a recombinant protein in frame with an N-terminal histidine tag followed by a PreScission protease cleavage site.
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Depositing Lab
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Depositing OrganizationNational Cancer Institute (NCI)
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 260902 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepRSET
- Backbone size w/o insert (bp) 2897
- Total vector size (bp) 3410
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehRpn13 (1-150)
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SpeciesH. sapiens (human)
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Insert Size (bp)513
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GenBank IDNM_175573
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Tag
/ Fusion Protein
- N-terminal His-tag (N terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSET hRpn13 (1-150) was a gift from Kylie Walters (Addgene plasmid # 260902 ; http://n2t.net/addgene:260902 ; RRID:Addgene_260902) -
For your References section:
Structure-guided bifunctional molecules hit a DEUBAD-lacking hRpn13 species upregulated in multiple myeloma. Lu X, Sabbasani VR, Osei-Amponsa V, Evans CN, King JC, Tarasov SG, Dyba M, Das S, Chan KC, Schwieters CD, Choudhari S, Fromont C, Zhao Y, Tran B, Chen X, Matsuo H, Andresson T, Chari R, Swenson RE, Tarasova NI, Walters KJ. Nat Commun. 2021 Dec 16;12(1):7318. doi: 10.1038/s41467-021-27570-4. 10.1038/s41467-021-27570-4 PubMed 34916494