Structure-guided bifunctional molecules hit a DEUBAD-lacking hRpn13 species upregulated in multiple myeloma.

Lu X, Sabbasani VR, Osei-Amponsa V, Evans CN, King JC, Tarasov SG, Dyba M, Das S, Chan KC, Schwieters CD, Choudhari S, Fromont C, Zhao Y, Tran B, Chen X, Matsuo H, Andresson T, Chari R, Swenson RE, Tarasova NI, Walters KJ
Nat Commun. 2021 Dec 16;12(1):7318. doi: 10.1038/s41467-021-27570-4. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
260902	pRSET hRpn13 (1-150)	hRpn13 Pru (1–150) was expressed in E. coli BL21(DE3) pLysS cells as a recombinant protein in frame with an N-terminal histidine tag followed by a PreScission protease cleavage site.