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CaV1.2
(Plasmid #26572)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26572 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 26572-D50 50 μg of DNA in Tris buffer $495
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DNA 26572-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA6/V5-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10F'
  • Growth instructions
    Top10F'
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Calcium channel, voltage-dependent, L type, alpha 1C subunit
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6600
  • Entrez Gene
    Cacna1c (a.k.a. Cav1.2, Cchl1a1, D930026N18Rik, MBC, MELC-CC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank Accesssion number: AY728090

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaV1.2 was a gift from Diane Lipscombe (Addgene plasmid # 26572 ; http://n2t.net/addgene:26572 ; RRID:Addgene_26572)

  • For your References section:

    Neuronal L-type calcium channels open quickly and are inhibited slowly. Helton TD, Xu W, Lipscombe D. J Neurosci. 2005 Nov 2. 25(44):10247-51. 10.1523/JNEUROSCI.1089-05.2005 PubMed 16267232

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