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Depositing Lab
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Depositing OrganizationBrown University
Located in the United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26573 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 26573-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 26573-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCR3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5060
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10F'
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Growth instructionsTop10F'
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCacna1a
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Alt namecalcium channel, voltage-dependent, P/Q type, alpha 1A subunit
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SpeciesM. musculus (mouse)
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Insert Size (bp)7100
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Entrez GeneCacna1a (a.k.a. APCA, BI, Caca1a, Cacnl1a4, Cav2.1, Ccha1a, EA2, FHM, HPCA, MHP, MHP1, SCA6, alpha1A, la, nmf352, rkr, smrl, tg)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The fragment was amplified from mouse brain. The GenBank sequence is AY714490.
DNA (Catalog # 26573-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 26573-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CaV2.1 (pCR3.1) was a gift from Diane Lipscombe (Addgene plasmid # 26573 ; http://n2t.net/addgene:26573 ; RRID:Addgene_26573) -
For your References section:
Novel CaV2.1 clone replicates many properties of Purkinje cell CaV2.1 current. Richards KS, Swensen AM, Lipscombe D, Bommert K. Eur J Neurosci. 2007 Nov . 26(10):2950-61. 10.1111/j.1460-9568.2007.05912.x PubMed 18001290