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pCAG-YTB
(Plasmid #26721)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 26721 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 26721-D50 50 μg of DNA in Tris buffer $495
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DNA 26721-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCAG-venusYFP
  • Backbone size w/o insert (bp) 3520
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    venusYFP, TVA950, Rabies Glycoprotein
  • Alt name
    TVA
  • Alt name
    RABVgp4
  • Alt name
    EnvA
  • Species
    G. gallus (chicken); Rabies virus (strain SAD B19)
  • Insert Size (bp)
    2901
  • GenBank ID
    M31046
  • Entrez Gene
    RABVgp4 (a.k.a. RABVgp4)
  • Entrez Gene
    CD320 (a.k.a. TVA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site StuI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene sequencing result shows a CMV promoter is present. A CAG promoter was not identified as the plasmid name implies.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-YTB was a gift from Edward Callaway (Addgene plasmid # 26721 ; http://n2t.net/addgene:26721 ; RRID:Addgene_26721)

  • For your References section:

    Targeting single neuronal networks for gene expression and cell labeling in vivo. Marshel JH, Mori T, Nielsen KJ, Callaway EM. Neuron. 2010 Aug 26. 67(4):562-74. 10.1016/j.neuron.2010.08.001 PubMed 20797534
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