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p3xFLAG-CMV-Tlr4
(Plasmid #27148)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27148 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 27148-D50 50 μg of DNA in Tris buffer $495
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DNA 27148-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    p3xFLAG-CMV-8
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4732
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP 10, regular growth condition.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    toll-like receptor 4
  • Alt name
    Tlr4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2436
  • Mutation
    Native signal peptide sequence (original amino acids 1-24) was removed for cloning.
  • GenBank ID
    NM_021297
  • Entrez Gene
    Tlr4 (a.k.a. Lps, Ly87, Ran/M1, Rasl2-8)
  • Tag / Fusion Protein
    • 3x FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV 30 sequencing primer
  • 3′ sequencing primer CMV 24 sequencing primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-CMV-Tlr4 was a gift from Bruce Beutler (Addgene plasmid # 27148 ; http://n2t.net/addgene:27148 ; RRID:Addgene_27148)

  • For your References section:

    Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, Birdwell D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli P, Layton B, Beutler B. Science. 1998 Dec 11. 282(5396):2085-8. 10.1126/science.282.5396.2085 PubMed 9851930
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