p3xFLAG-CMV-Tlr4(Lps)
(Plasmid
#27149)
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Depositing Lab
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Depositing OrganizationScripps California (The Scripps Research Institute)
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27149 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 27149-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 27149-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonep3xFLAG-CMV-8
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4732
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10 under regular growing condition
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametoll-like receptor 4(Lps)
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Alt nameTlr4(Lps)
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SpeciesM. musculus (mouse)
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Insert Size (bp)2436
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Mutationchanged Proline 712 to Histidine. Native signal peptide sequence (original amino acids 1-24) was removed for cloning.
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GenBank IDNM_021297
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Entrez GeneTlr4 (a.k.a. Lps, Ly87, Ran/M1, Rasl2-8)
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Tag
/ Fusion Protein
- 3x FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV30 sequencing primer
- 3′ sequencing primer CMV 24 sequencing primer
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 27149-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 27149-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3xFLAG-CMV-Tlr4(Lps) was a gift from Bruce Beutler (Addgene plasmid # 27149 ; http://n2t.net/addgene:27149 ; RRID:Addgene_27149) -
For your References section:
Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, Birdwell D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli P, Layton B, Beutler B. Science. 1998 Dec 11. 282(5396):2085-8. 10.1126/science.282.5396.2085 PubMed 9851930