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pDEST/N1-hBIN1-GFP
(Plasmid #27305)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27305 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 27305-D50 50 μg of DNA in Tris buffer $495
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DNA 27305-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pDEST/N1-GFP
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bridging integrator 1 (BIN1), transcript variant 8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1362
  • Entrez Gene
    BIN1 (a.k.a. AMPH2, AMPHL, CNM2, SH3P9)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 gateway reaction site (not destroyed)
  • 3′ cloning site attR2 gateway reaction site (not destroyed)
  • 5′ sequencing primer sequence
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We did not originally clone this gene. We ordered human BIN1 (transcript variance 8) from origene and cloned it into our gateway destination vector pDEST/N1-GFP. The N1-GFP vector is originally ordered from Clonetech and we converted it into a gateway destination vector.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST/N1-hBIN1-GFP was a gift from Robin Shaw (Addgene plasmid # 27305 ; http://n2t.net/addgene:27305 ; RRID:Addgene_27305)

  • For your References section:

    BIN1 localizes the L-type calcium channel to cardiac T-tubules. Hong TT, Smyth JW, Gao D, Chu KY, Vogan JM, Fong TS, Jensen BC, Colecraft HM, Shaw RM. PLoS Biol. 2010 Feb . 8(2):e1000312. 10.1371/journal.pbio.1000312 PubMed 20169111
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