pcDNA3.2-hBIN1-V5
(Plasmid #27303)

• Depositing Lab: Robin Shaw
• Publication: Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

Backbone

• Vector backbone: pcDNA3.2/V5-Dest (Invitrogen)
• Backbone size w/o insert (bp): 7711
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: bridging integrator 1 (BIN1), transcript variant 8
• Species: H. sapiens (human)
• Insert Size (bp): 1362
• GenBank ID: NM_004305
• Entrez Gene: BIN1 (a.k.a. AMPH2, AMPHL, CNM2, SH3P9)
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR1 gateway reaction site (not destroyed)
• 3′ cloning site: attR2 gateway reaction site (not destroyed)
• 5′ sequencing primer: sequence

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We did not originally clone this gene. We ordered human BIN1 (transcript variance 8)from Origene and cloned it into the gateway backbone of pcDNA3.2/V5-Dest.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry