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CAV1-mEGFP-mCherry
(Plasmid #27763)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27763 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 27763-D50 50 μg of DNA in Tris buffer $495
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DNA 27763-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5069
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    caveolin-1
  • Alt name
    CAV1
  • Alt name
    cav-1
  • Species
    Canis lupus familiaris
  • Insert Size (bp)
    2034
  • GenBank ID
    NM_001003296
  • Entrez Gene
    CAV1
  • Tag / Fusion Protein
    • monomeric EGFP-monomeric Cherry (tandem) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pcDNA5/FRT/TO backbone is compatible with Flp-In recombination for generation of stable cell lines and/or tet-inducible expression. Can also be used for transient expression.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAV1-mEGFP-mCherry was a gift from Ari Helenius (Addgene plasmid # 27763 ; http://n2t.net/addgene:27763 ; RRID:Addgene_27763)

  • For your References section:

    Caveolin-1 is ubiquitinated and targeted to intralumenal vesicles in endolysosomes for degradation. Hayer A, Stoeber M, Ritz D, Engel S, Meyer HH, Helenius A. J Cell Biol. 2010 Nov 1. 191(3):615-29. 10.1083/jcb.201003086 PubMed 21041450
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