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CAV1-mCherry
(Plasmid #27705)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27705 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3961
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    caveolin-1
  • Alt name
    CAV1, cav-1
  • Species
    Canis lupus familiaris
  • Insert Size (bp)
    1313
  • GenBank ID
    NM_001003296
  • Entrez Gene
    CAV1 (a.k.a. caveolin 1)
  • Tag / Fusion Protein
    • monomeric Cherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer ACC TCT ACA AAT GTG GTA TGG CTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAV1-mCherry was a gift from Ari Helenius (Addgene plasmid # 27705 ; http://n2t.net/addgene:27705 ; RRID:Addgene_27705)
  • For your References section:

    Biogenesis of caveolae: stepwise assembly of large caveolin and cavin complexes. Hayer A, Stoeber M, Bissig C, Helenius A. Traffic. 2010 Mar . 11(3):361-82. 10.1111/j.1600-0854.2009.01023.x PubMed 20070607