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mRFP-Clc
(Plasmid #14435)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14435 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmRFP-C3
  • Backbone size w/o insert (bp) 4668
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Clathrin light chain
  • Alt name
    Clc
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    714
  • GenBank ID
  • Entrez Gene
    Clc (a.k.a. Clcp, gal-10)
  • Tag / Fusion Protein
    • mRFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer 5'-GACTACACCATCGTGGAA
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was based on the EGFP-Clta plasmid from James Keen (http://www.nature.com/ncb/journal/v1/n1/pdf/ncb0599_1.pdf), which was generated from a mouse placental cDNA clone
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

This plasmid contains clathrin light chain A, isoform a, with an A9D mutation relative to NM_001080384 (NP_001073853). This mutation, however, seems to be a natural variant, as it is present in other published sequences for mouse Clta

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRFP-Clc was a gift from Ari Helenius (Addgene plasmid # 14435 ; http://n2t.net/addgene:14435 ; RRID:Addgene_14435)
  • For your References section:

    Assembly and trafficking of caveolar domains in the cell: caveolae as stable, cargo-triggered, vesicular transporters. Tagawa A, Mezzacasa A, Hayer A, Longatti A, Pelkmans L, Helenius A. J Cell Biol. 2005 Aug 29. 170(5):769-79. 10.1083/jcb.200506103 PubMed 16129785