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Depositing Lab
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Depositing OrganizationETH Zurich (Swiss Federal Institute of Technology)
Located in Switzerland -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14435 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 14435-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 14435-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepmRFP-C3
- Backbone size w/o insert (bp) 4668
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameClathrin light chain
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Alt nameClc
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SpeciesM. musculus (mouse)
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Insert Size (bp)714
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GenBank ID
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Entrez GeneClc (a.k.a. Clcp, gal-10)
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Tag
/ Fusion Protein
- mRFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer 5'-GACTACACCATCGTGGAA
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis plasmid was based on the EGFP-Clta plasmid from James Keen (http://www.nature.com/ncb/journal/v1/n1/pdf/ncb0599_1.pdf), which was generated from a mouse placental cDNA clone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains clathrin light chain A, isoform a, with an A9D mutation relative to NM_001080384 (NP_001073853). This mutation, however, seems to be a natural variant, as it is present in other published sequences for mouse Clta
DNA (Catalog # 14435-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 14435-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mRFP-Clc was a gift from Ari Helenius (Addgene plasmid # 14435 ; http://n2t.net/addgene:14435 ; RRID:Addgene_14435) -
For your References section:
Assembly and trafficking of caveolar domains in the cell: caveolae as stable, cargo-triggered, vesicular transporters. Tagawa A, Mezzacasa A, Hayer A, Longatti A, Pelkmans L, Helenius A. J Cell Biol. 2005 Aug 29. 170(5):769-79. 10.1083/jcb.200506103 PubMed 16129785
