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Lamp1-RFP
(Plasmid #1817)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1817 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Modified Clontech Plasmid
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lysosome associated membrane protein 1
  • Alt name
    Lamp-1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1200
  • Mutation
    D50E (not important for function of plasmid)
  • Entrez Gene
    Lamp1 (a.k.a. LGP120)
  • Tag / Fusion Protein
    • RFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer DsRed1-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Lamp-1 sequence contains a D50E mutation, which is not important for function of plasmid. There is also a silent mutation at bp#715 compared to GenBank NM_012857.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lamp1-RFP was a gift from Walther Mothes (Addgene plasmid # 1817 ; http://n2t.net/addgene:1817 ; RRID:Addgene_1817)
  • For your References section:

    Visualization of retroviral replication in living cells reveals budding into multivesicular bodies. Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W. Traffic 2003 Nov;4(11):785-801. 10.1034/j.1600-0854.2003.00135.x PubMed 14617360