pmRFP-LC3
(Plasmid #21075)

• Purpose: Mammalian expression of rat LC3 fused to mRFP
• Depositing Lab: Tamotsu Yoshimori
• Publication: Kimura et al Autophagy. 2007;3(5):452-60.

Backbone

• Vector backbone: pmRFP-C1
  (Search Vector Database)
• Backbone size w/o insert (bp): 4500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: microtubule-associated protein 1 light chain 3 beta
• Alt name: Map1lc3b
• Alt name: LC3
• Alt name: LC3b
• Species: R. norvegicus (rat)
• Insert Size (bp): 738
• Mutation: First ATG is not included.
• GenBank ID: U05784
• Entrez Gene: Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
• Tag / Fusion Protein:
  • mRFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 72 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharian DA, Tsien RY, A monomeric red fluorescent protein, Proc Natl Acad Sci USA. 2002, 99(12):7877-82