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pmCherry-ATG5
(Plasmid #13095)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13095 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFPC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3941
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATG5
  • Alt name
    ATG5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1576
  • GenBank ID
    AB048349
  • Entrez Gene
    Atg5 (a.k.a. 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer NA
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

for mCherry marker protein
Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharias DA,
Tsien RY. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-ATG5 was a gift from Roberta Gottlieb (Addgene plasmid # 13095 ; http://n2t.net/addgene:13095 ; RRID:Addgene_13095)
  • For your References section:

    Response to myocardial ischemia/reperfusion injury involves Bnip3 and autophagy. Hamacher-Brady A, Brady NR, Logue SE, Sayen MR, Jinno M, Kirshenbaum LA, Gottlieb RA, Gustafsson AB. Cell Death Differ. 2006 Apr 28. ():. 10.1038/sj.cdd.4401936 PubMed 16645637