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ptfLC3
(Plasmid #21074)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21074 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1+mRFP
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    microtubule-associated protein 1 light chain 3 beta
  • Alt name
    Map1lc3b
  • Alt name
    LC3
  • Alt name
    LC3b
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    738
  • GenBank ID
    U05784
  • Entrez Gene
    Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
  • Tags / Fusion Proteins
    • mRFP (N terminal on backbone)
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Compbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharian DA, Tsien RY, A monomeric red fluorescent protein, Proc Natl Acad Sci USA. 2002, 99(12):7877-82

NOTE FROM AUTHORS: We found several alterations between our plasmid and the version on NCBI [Map1lc3b; https://www.ncbi.nlm.nih.gov/gene/64862) ].
Our original clone may be a kind of variant.
In the C-terminal end of tfLC3, the stop codon is removed, which causes an additional 17 amino acid residues.
However, the C-terminal end from Gly120, including the 17 amino acids, is immediately cut off by Atg4 protease after the translation.
Therefore that sequence variant will not affect the function of the protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ptfLC3 was a gift from Tamotsu Yoshimori (Addgene plasmid # 21074 ; http://n2t.net/addgene:21074 ; RRID:Addgene_21074)
  • For your References section:

    Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Kimura S, Noda T, Yoshimori T. Autophagy. . 3(5):452-60. PubMed 17534139