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(Plasmid #21074)


Item Catalog # Description Quantity Price (USD)
Plasmid 21074 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    microtubule-associated protein 1 light chain 3 beta
  • Alt name
  • Alt name
  • Alt name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
  • Tags / Fusion Proteins
    • mRFP (N terminal on backbone)
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Compbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharian DA, Tsien RY, A monomeric red fluorescent protein, Proc Natl Acad Sci USA. 2002, 99(12):7877-82

NOTE FROM AUTHORS: We found several alterations between our plasmid and the version on NCBI [Map1lc3b; ].
Our original clone may be a kind of variant.
In the C-terminal end of tfLC3, the stop codon is removed, which causes an additional 17 amino acid residues.
However, the C-terminal end from Gly120, including the 17 amino acids, is immediately cut off by Atg4 protease after the translation.
Therefore that sequence variant will not affect the function of the protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ptfLC3 was a gift from Tamotsu Yoshimori (Addgene plasmid # 21074 ; ; RRID:Addgene_21074)
  • For your References section:

    Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Kimura S, Noda T, Yoshimori T. Autophagy. 2007;3(5):452-60. 10.4161/auto.4451 PubMed 17534139