PurposeMammalian expression of rat LC3 fused to mRFP and EGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21074||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Gene/Insert namemicrotubule-associated protein 1 light chain 3 beta
SpeciesR. norvegicus (rat)
Insert Size (bp)738
Entrez GeneMap1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
/ Fusion Proteins
- mRFP (N terminal on backbone)
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Compbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharian DA, Tsien RY, A monomeric red fluorescent protein, Proc Natl Acad Sci USA. 2002, 99(12):7877-82
NOTE FROM AUTHORS: We found several alterations between our plasmid and the version on NCBI [Map1lc3b; https://www.ncbi.nlm.nih.gov/gene/64862) ].
Our original clone may be a kind of variant.
In the C-terminal end of tfLC3, the stop codon is removed, which causes an additional 17 amino acid residues.
However, the C-terminal end from Gly120, including the 17 amino acids, is immediately cut off by Atg4 protease after the translation.
Therefore that sequence variant will not affect the function of the protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ptfLC3 was a gift from Tamotsu Yoshimori (Addgene plasmid # 21074 ; http://n2t.net/addgene:21074 ; RRID:Addgene_21074)
For your References section:Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Kimura S, Noda T, Yoshimori T. Autophagy. 2007;3(5):452-60. 10.4161/auto.4451 PubMed 17534139