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pBABE-puro mCherry-EGFP-LC3B
(Plasmid #22418)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22418 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    microtubule-associated protein 1 light chain 3 beta
  • Alt name
    MAP1LC3B
  • Alt name
    LC3B
  • Alt name
    LC3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • GenBank ID
    BC045759
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please click the "Notes from Addgene" link above for sequence submitted by a requesting scientist and further observations on using this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-puro mCherry-EGFP-LC3B was a gift from Jayanta Debnath (Addgene plasmid # 22418 ; http://n2t.net/addgene:22418 ; RRID:Addgene_22418)
  • For your References section:

    PLIC proteins or ubiquilins regulate autophagy-dependent cell survival during nutrient starvation. N'Diaye EN, Kajihara KK, Hsieh I, Morisaki H, Debnath J, Brown EJ. EMBO Rep. 2009 Feb;10(2):173-9. doi: 10.1038/embor.2008.238. Epub 2009 Jan 16. 10.1038/embor.2008.238 PubMed 19148225