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Cav1-mRFP
(Plasmid #14434)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14434 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 14434-D50 50 μg of DNA in Tris buffer $495
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DNA 14434-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmRFP-N1
  • Backbone size w/o insert (bp) 4687
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Caveolin-1
  • Alt name
    Cav1
  • Species
    Canis familalis
  • Insert Size (bp)
    534
  • GenBank ID
    NM_001003296
  • Entrez Gene
    CAV1
  • Tag / Fusion Protein
    • mRFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (destroyed during cloning)
  • 5′ sequencing primer 5'-CCAAAATGTCGTAACAACTCCG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the depositing laboratory recommends using CAV1-mCherry (Addgene Plasmid #27705) instead of CAV1-mRFP due to improved properties of the mCherry fluorophore.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cav1-mRFP was a gift from Ari Helenius (Addgene plasmid # 14434 ; http://n2t.net/addgene:14434 ; RRID:Addgene_14434)

  • For your References section:

    Assembly and trafficking of caveolar domains in the cell: caveolae as stable, cargo-triggered, vesicular transporters. Tagawa A, Mezzacasa A, Hayer A, Longatti A, Pelkmans L, Helenius A. J Cell Biol. 2005 Aug 29. 170(5):769-79. 10.1083/jcb.200506103 PubMed 16129785
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