|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14434||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4687
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)534
Entrez GeneCAV1 (a.k.a. caveolin 1)
/ Fusion Protein
- mRFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (destroyed during cloning)
- 5′ sequencing primer 5'-CCAAAATGTCGTAACAACTCCG (Common Sequencing Primers)
Please note that the depositing laboratory recommends using CAV1-mCherry (Addgene Plasmid #27705) instead of CAV1-mRFP due to improved properties of the mCherry fluorophore.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Cav1-mRFP was a gift from Ari Helenius (Addgene plasmid # 14434 ; http://n2t.net/addgene:14434 ; RRID:Addgene_14434)
For your References section:Assembly and trafficking of caveolar domains in the cell: caveolae as stable, cargo-triggered, vesicular transporters. Tagawa A, Mezzacasa A, Hayer A, Longatti A, Pelkmans L, Helenius A. J Cell Biol. 2005 Aug 29. 170(5):769-79. 10.1083/jcb.200506103 PubMed 16129785