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pEYFP-C3-HA-Ubiquitin
(Plasmid #28236)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28236 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 28236-D50 50 μg of DNA in Tris buffer $495
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DNA 28236-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEYFP-C3
  • Backbone size w/o insert (bp) 4727
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha, XL10 gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin
  • Alt name
    UBB, UBC, UBA52, RPS27A, MGC8385, FLJ25987
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    274
  • GenBank ID
    AB089617
  • Entrez Gene
    UBB (a.k.a. HEL-S-50)
  • Tags / Fusion Proteins
    • EYFP (N terminal on backbone)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    E.T.H. Yeh & T. Kamitani

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene Plasmid 18712: HA-Ubiquitin

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEYFP-C3-HA-Ubiquitin was a gift from Michael Mancini (Addgene plasmid # 28236 ; http://n2t.net/addgene:28236 ; RRID:Addgene_28236)

  • For your References section:

    Intranuclear ataxin1 inclusions contain both fast- and slow-exchanging components. Stenoien DL, Mielke M, Mancini MA. Nat Cell Biol. 2002 Oct . 4(10):806-10. 10.1038/ncb859 PubMed 12360291
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