PurposeMammalian expression of human ubiquitin with HA tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18712||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
MutationThe HA-Ubiquitin insert is from the UBB gene. The reason for the small size is because it contains a single ubiquitin coding sequence(full length UBB has three repeating ubiquitin coding sequences).
Entrez GeneUBB (a.k.a. HEL-S-50)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
This plasmid grows slowly. We encourage requesting scientists to grow for a longer period of time than normal.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HA-Ubiquitin was a gift from Edward Yeh (Addgene plasmid # 18712 ; http://n2t.net/addgene:18712 ; RRID:Addgene_18712)
For your References section:Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein. Kamitani T, Kito K, Nguyen HP, Yeh ET. J Biol Chem. 1997 Nov 7. 272(45):28557-62. 10.1074/jbc.272.45.28557 PubMed 9353319