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HA-Ubiquitin
(Plasmid #18712)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18712 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ubiquitin
  • Species
    H. sapiens (human)
  • Mutation
    The HA-Ubiquitin insert is from the UBB gene. The reason for the small size is because it contains a single ubiquitin coding sequence(full length UBB has three repeating ubiquitin coding sequences).
  • GenBank ID
    AB089617
  • Entrez Gene
    UBB (a.k.a. HEL-S-50)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid grows slowly. We encourage requesting scientists to grow for a longer period of time than normal.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-Ubiquitin was a gift from Edward Yeh (Addgene plasmid # 18712 ; http://n2t.net/addgene:18712 ; RRID:Addgene_18712)
  • For your References section:

    Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein. Kamitani T, Kito K, Nguyen HP, Yeh ET. J Biol Chem. 1997 Nov 7. 272(45):28557-62. 10.1074/jbc.272.45.28557 PubMed 9353319