PurposeMammalian expression of human Sumo1 with HA tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21154||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepcDNA3.1 HA
Modifications to backboneBGH polyA terminator removed during cloning of Sumo1
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
- Promoter CMV
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
This plasmid does not contain the BGH polyA terminator present in the standard pcDNA3 backbone. It is not known whether the removal of the terminator affects expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-HA-Sumo1 was a gift from Junying Yuan (Addgene plasmid # 21154 ; http://n2t.net/addgene:21154 ; RRID:Addgene_21154)
For your References section:Dual role of sumoylation in the nuclear localization and transcriptional activation of NFAT1. Terui Y, Saad N, Jia S, McKeon F, Yuan J. J Biol Chem. 2004 Jul 2. 279(27):28257-65. 10.1074/jbc.M403153200 PubMed 15117942