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pcDNA3-HA-Sumo1
(Plasmid #21154)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21154 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 HA
  • Modifications to backbone
    BGH polyA terminator removed during cloning of Sumo1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pcDNA3-HA-Sumo1
  • Alt name
    Sumo1
  • Species
    H. sapiens (human)
  • Entrez Gene
    SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid does not contain the BGH polyA terminator present in the standard pcDNA3 backbone. It is not known whether the removal of the terminator affects expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-Sumo1 was a gift from Junying Yuan (Addgene plasmid # 21154 ; http://n2t.net/addgene:21154 ; RRID:Addgene_21154)
  • For your References section:

    Dual role of sumoylation in the nuclear localization and transcriptional activation of NFAT1. Terui Y, Saad N, Jia S, McKeon F, Yuan J. J Biol Chem. 2004 Jul 2. 279(27):28257-65. 10.1074/jbc.M403153200 PubMed 15117942