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p3258 pCMV hUBC9 wt HA
(Plasmid #14438)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14438 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 14438-D50 50 μg of DNA in Tris buffer $495
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DNA 14438-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV4
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBC9
  • Alt name
    UBC 9
  • Alt name
    hUBC9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • Entrez Gene
    UBE2I (a.k.a. C358B7.1, P18, UBC9)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BclI/BglII (destroyed during cloning)
  • 5′ sequencing primer CMV forward
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A fragment containing the full coding region of hubc9 cDNA along with the influenza virus hemagglutinin 1 epitope (HA) sequence (YPYDVPDYA) at the 3′-end was produced with appropriate synthetic oligonucleotides using PCR. The fragment was then cloned into the pCMV4 vector at the BglII site.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3258 pCMV hUBC9 wt HA was a gift from Peter Howley (Addgene plasmid # 14438 ; http://n2t.net/addgene:14438 ; RRID:Addgene_14438)

  • For your References section:

    Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9. Yasugi T, Howley PM. Nucleic Acids Res. 1996 Jun 1. 24(11):2005-10. 10.1093/nar/24.11.2005 PubMed 8668529
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