p3258 pCMV hUBC9 wt HA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14438||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameUBC 9
SpeciesH. sapiens (human)
Insert Size (bp)500
Entrez GeneUBE2I (a.k.a. C358B7.1, P18, UBC9)
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BclI/BglII (destroyed during cloning)
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
A fragment containing the full coding region of hubc9 cDNA along with the influenza virus hemagglutinin 1 epitope (HA) sequence (YPYDVPDYA) at the 3′-end was produced with appropriate synthetic oligonucleotides using PCR. The fragment was then cloned into the pCMV4 vector at the BglII site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3258 pCMV hUBC9 wt HA was a gift from Peter Howley (Addgene plasmid # 14438 ; http://n2t.net/addgene:14438 ; RRID:Addgene_14438)
For your References section:Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9. Yasugi T, Howley PM. Nucleic Acids Res. 1996 Jun 1. 24(11):2005-10. 10.1093/nar/24.11.2005 PubMed 8668529