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SRa-HA-SUMO2 (Sentrin 3)
(Plasmid #17360)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17360 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUMO2
  • Alt name
    Sentrin3
  • Species
    H. sapiens (human)
  • Entrez Gene
    SUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SRa-HA-SUMO2 (Sentrin 3) was a gift from Edward Yeh (Addgene plasmid # 17360 ; http://n2t.net/addgene:17360 ; RRID:Addgene_17360)
  • For your References section:

    Covalent modification of PML by the sentrin family of ubiquitin-like proteins. Kamitani T, Nguyen HP, Kito K, Fukuda-Kamitani T, Yeh ET. J Biol Chem. 1998 Feb 6. 273(6):3117-20. 10.1074/jbc.273.6.3117 PubMed 9452416