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p3259 pCMV hUBC9 mt HA
(Plasmid #14439)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14439 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBC9 C93S
  • Alt name
    UBC 9
  • Alt name
    hUBC9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • Mutation
    Cys93Ser mutation (TGC changed to AGC)
  • Entrez Gene
    UBE2I (a.k.a. C358B7.1, P18, UBC9)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BclI/BglII (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3')
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A fragment containing the full coding region of hubc9 C93S cDNA along with the influenza virus hemagglutinin 1 epitope (HA) sequence (YPYDVPDYA) at the 3′-end was produced with appropriate synthetic oligonucleotides using PCR. The fragment was then cloned into the pCMV4 vector at the BglII site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3259 pCMV hUBC9 mt HA was a gift from Peter Howley (Addgene plasmid # 14439 ; http://n2t.net/addgene:14439 ; RRID:Addgene_14439)
  • For your References section:

    Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9. Yasugi T, Howley PM. Nucleic Acids Res. 1996 Jun 1. 24(11):2005-10. 10.1093/nar/24.11.2005 PubMed 8668529