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(Plasmid #29559)


Item Catalog # Description Quantity Price (USD)
Plasmid 29559 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    centrin 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    CETN2 (a.k.a. CALT, CEN2)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site XmaI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Centrin-2 sequence in this plasmid contains an additional serine at position 3 when compared to GenBank NP_004335.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCent2-pEGFP-C1 was a gift from Jeffrey Salisbury (Addgene plasmid # 29559 ; ; RRID:Addgene_29559)
  • For your References section:

    Centrin-2 is required for centriole duplication in mammalian cells. Salisbury JL, Suino KM, Busby R, Springett M. Curr Biol. 2002 Aug 6. 12(15):1287-92. 10.1016/S0960-9822(02)01019-9 PubMed 12176356