This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

hCent2-pEGFP-C1
(Plasmid #29559)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29559 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cent2
  • Alt name
    centrin 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    516
  • Entrez Gene
    CETN2 (a.k.a. CALT, CEN2)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site XmaI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Centrin-2 sequence in this plasmid contains an additional serine at position 3 when compared to GenBank NP_004335.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCent2-pEGFP-C1 was a gift from Jeffrey Salisbury (Addgene plasmid # 29559 ; http://n2t.net/addgene:29559 ; RRID:Addgene_29559)
  • For your References section:

    Centrin-2 is required for centriole duplication in mammalian cells. Salisbury JL, Suino KM, Busby R, Springett M. Curr Biol. 2002 Aug 6. 12(15):1287-92. 10.1016/S0960-9822(02)01019-9 PubMed 12176356