mCh-alpha-tubulin
(Plasmid #49149)

• Purpose: expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.
• Depositing Lab: Gia Voeltz
• Publication: Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. doi: 10.1083/jcb.200911024.

Backbone

• Vector backbone: EYFP-alpha-tubulin
• Backbone manufacturer: Takara Bio Inc
• Modifications to backbone: mCherry substituted for EYFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: alpha tubulin
• Alt name: tubulin, alpha 1b
• Alt name: α tubulin
• Alt name: TUBA1B
• Species: H. sapiens (human)
• Entrez Gene: TUBA1B (a.k.a. K-ALPHA-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: mCherry-F (ccccgtaatgcagaagaaga)

Resource Information

• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry