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mCh-alpha-tubulin
(Plasmid #49149)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49149 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    EYFP-alpha-tubulin
  • Backbone manufacturer
    Takara Bio Inc
  • Modifications to backbone
    mCherry substituted for EYFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alpha tubulin
  • Alt name
    tubulin, alpha 1b
  • Alt name
    α tubulin
  • Alt name
    TUBA1B
  • Species
    H. sapiens (human)
  • Entrez Gene
    TUBA1B (a.k.a. K-ALPHA-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCh-alpha-tubulin was a gift from Gia Voeltz (Addgene plasmid # 49149 ; http://n2t.net/addgene:49149 ; RRID:Addgene_49149)
  • For your References section:

    ER sliding dynamics and ER-mitochondrial contacts occur on acetylated microtubules. Friedman JR, Webster BM, Mastronarde DN, Verhey KJ, Voeltz GK. J Cell Biol. 2010 Aug 9;190(3):363-75. doi: 10.1083/jcb.200911024. 10.1083/jcb.200911024 PubMed 20696706