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Addgene

mCh-Rab5
(Plasmid #49201)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49201 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCh-alpha tubulin (Addgene plasmid 49149)
  • Backbone manufacturer
    Voeltz lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab5
  • Alt name
    RAB5B, member RAS oncogene family
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAB5B
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCh-Rab5 was a gift from Gia Voeltz (Addgene plasmid # 49201 ; http://n2t.net/addgene:49201 ; RRID:Addgene_49201)

  • For your References section:

    ER sliding dynamics and ER-mitochondrial contacts occur on acetylated microtubules. Friedman JR, Webster BM, Mastronarde DN, Verhey KJ, Voeltz GK. J Cell Biol. 2010 Aug 9;190(3):363-75. doi: 10.1083/jcb.200911024. 10.1083/jcb.200911024 PubMed 20696706
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