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mCh-Rab7A
(Plasmid #61804)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61804 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAcGFP1-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5324
  • Modifications to backbone
    mCh tag was amplified and inserted into the NheI and XhoI sites in order to replace the GFP.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab7A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    624
  • Mutation
    None
  • GenBank ID
    NM_004637.5
  • Entrez Gene
    RAB7A (a.k.a. CMT2B, PRO2706, RAB7)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCh (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CCA TCG TGG AAC AGT ACG AACG
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCh-Rab7A was a gift from Gia Voeltz (Addgene plasmid # 61804 ; http://n2t.net/addgene:61804 ; RRID:Addgene_61804)
  • For your References section:

    ER Contact Sites Define the Position and Timing of Endosome Fission. Rowland AA, Chitwood PJ, Phillips MJ, Voeltz GK. Cell. 2014 Nov 20;159(5):1027-41. doi: 10.1016/j.cell.2014.10.023. 10.1016/j.cell.2014.10.023 PubMed 25416943