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pLAMP1-mCherry
(Plasmid #45147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7500
  • Modifications to backbone
    modified to contain mCherry and be compatible with Gateway cloning
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LAMP1-mCherry
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2000
  • Entrez Gene
    LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLAMP1-mCherry was a gift from Amy Palmer (Addgene plasmid # 45147 ; http://n2t.net/addgene:45147 ; RRID:Addgene_45147)

  • For your References section:

    Imaging type-III secretion reveals dynamics and spatial segregation of Salmonella effectors. Van Engelenburg SB, Palmer AE. Nat Methods. 2010 Apr;7(4):325-30. doi: 10.1038/nmeth.1437. Epub 2010 Mar 14. 10.1038/nmeth.1437 PubMed 20228815
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