pAd/CMV/DAX1-IRES-nEGFP
(Plasmid #29752)

• Depositing Lab: Edward McCabe
• Depositing Organization: University of California, Los Angeles (UCLA)
  Located in United States of America
• Publication: McCabe Lab Plasmids (unpublished)

Backbone

• Vector backbone: pAd/CMV/V5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 36686
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1
• Alt name: DAX1
• Alt name: NR0B1
• Species: H. sapiens (human)
• Insert Size (bp): 2840
• Mutation: No mutations; Reporter is nuclear localized EGFP; DAX1 and nEGFP is separated with an IRES
• GenBank ID: BC011564
• Entrez Gene: NR0B1 (a.k.a. AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1, SRXY2)
• Tags / Fusion Proteins:
  • IRES
  • Nuclear localization sequence
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attB1 (not destroyed)
• 3′ cloning site: attB2 (not destroyed)
• 5′ sequencing primer: attB1
• 3′ sequencing primer: V5

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Thermo Scientific/OpenBiosystems

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene distributes this plasmid in DH5a, but the depositing lab maintained the plasmid in OmniMAX 2 T1R (Invitrogen catalogue #C8540-03)

DNA (Catalog # 29752-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 29752-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.