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pAd/WT1-IRES-nAmCyan
(Plasmid #29756)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29756 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAd/CMV/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 36686
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Wilms tumor 1
  • Alt name
    WT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • Mutation
    No mutations; AmCyan is nuclear localized; WT1 and nAmCyan are separated by IRES; AmCyan from Clontech
  • GenBank ID
    BC032861
  • Entrez Gene
    WT1 (a.k.a. AWT1, GUD, NPHS4, WAGR, WIT-2, WT-1, WT33)
  • Tags / Fusion Proteins
    • IRES
    • Nuclear localization sequence
    • AmCyan (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attB1 (not destroyed)
  • 3′ cloning site attB2 (not destroyed)
  • 5′ sequencing primer attB1
  • 3′ sequencing primer V5
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    WT1 - Thermo Scientific/OpenBiosystems AmCyan - Clontech
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene distributes this plasmid in DH5a, but the depositing lab maintained the plasmid in OmniMAX™ 2 T1R (Invitrogen catalogue #C8540-03)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAd/WT1-IRES-nAmCyan was a gift from Edward McCabe (Addgene plasmid # 29756 ; http://n2t.net/addgene:29756 ; RRID:Addgene_29756)