pBABEpuro HA Brca1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14999||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneBRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
pBABEpuro HABrca1 was made by digesting pCDNA3beta HABrca1 (R. Scully, et al., Cell 88, 265, 1997) with SalI and XhoI and inserting it into the SalI site of pBABEpuro.
The BRCA1 insert in this plasmid contains the following common polymorphisms when compared to GenBank reference sequence NP_009225.1: P871L, E1038G, K1182R, and S1613G. These SNPs were likely present in the original cDNA isolate.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABEpuro HA Brca1 was a gift from Stephen Elledge (Addgene plasmid # 14999 ; http://n2t.net/addgene:14999 ; RRID:Addgene_14999)
For your References section:Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Cortez D, Wang Y, Qin J, Elledge SJ. Science. 1999 Nov 5. 286(5442):1162-6. 10.1126/science.286.5442.1162 PubMed 10550055