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pCL-MFG-BRCA1
(Plasmid #12341)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12341 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCLMFG
  • Backbone manufacturer
    Verma Lab
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRCA1
  • Species
    H. sapiens (human)
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (partial digest) (not destroyed)
  • 3′ cloning site XbaI/BamHI (destroyed during cloning)
  • 5′ sequencing primer LXSN primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Full-length BRCA1 cDNA was lifted from this construct as a NcoI (partial digest)-XbaI/blunted fragment and inserted into NcoI (partial digest)-cut and BamHI-cut/blunted pCL-MFG retroviral vector.

Addgene's quality control sequence analysis identified a sequence discrepancy relative to Genbank reference sequence NM_007294, resulting in a P871L amino acid substitution in the encoded insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCL-MFG-BRCA1 was a gift from Inder Verma (Addgene plasmid # 12341 ; http://n2t.net/addgene:12341 ; RRID:Addgene_12341)
  • For your References section:

    BRCA1 is a cell cycle-regulated nuclear phosphoprotein. Ruffner H, Verma IM. Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7138-43. 10.1073/pnas.94.14.7138 PubMed 9207057