|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12341||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVerma Lab
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneBRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (partial digest) (not destroyed)
- 3′ cloning site XbaI/BamHI (destroyed during cloning)
- 5′ sequencing primer LXSN primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Full-length BRCA1 cDNA was lifted from this construct as a NcoI (partial digest)-XbaI/blunted fragment and inserted into NcoI (partial digest)-cut and BamHI-cut/blunted pCL-MFG retroviral vector.
Addgene's quality control sequence analysis identified a sequence discrepancy relative to Genbank reference sequence NM_007294, resulting in a P871L amino acid substitution in the encoded insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCL-MFG-BRCA1 was a gift from Inder Verma (Addgene plasmid # 12341 ; http://n2t.net/addgene:12341 ; RRID:Addgene_12341)
For your References section:BRCA1 is a cell cycle-regulated nuclear phosphoprotein. Ruffner H, Verma IM. Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7138-43. 10.1073/pnas.94.14.7138 PubMed 9207057