pcDNA3 236HSC WT (BRCA2)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16246||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)10000
Entrez GeneBRCA2 (a.k.a. BRCC2, BROVCA2, FACD, FAD, FAD1, FANCD, FANCD1, GLM3, PNCA2, XRCC11)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
To construct p236BRCA2, the pcDNA3 vector was first modified by inserting a 236-bp fragment of the 5' untranslated region of BRCA2 between the KpnI and NotI sites. The assembled full-length BRCA2 cDNA was then inserted at the XhoI site of this plasmid. The 5' UTR of BRCA2 was obtained by RT-PCR using primers 5'-GGTACCGGTG GCGCGAGCTT CTGA-3' and 5'-GCGGCCGCAA CTACGATATT CCTCCAAT-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 236HSC WT (BRCA2) was a gift from Mien-Chie Hung (Addgene plasmid # 16246 ; http://n2t.net/addgene:16246 ; RRID:Addgene_16246)
For your References section:Inhibition of cancer cell growth by BRCA2. Wang SC, Shao R, Pao AY, Zhang S, Hung MC, Su LK. Cancer Res. 2002 Mar 1. 62(5):1311-4. PubMed 11888897