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pcDNA3 236HSC WT (BRCA2)
(Plasmid #16246)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16246 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRCA2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    10000
  • Entrez Gene
    BRCA2 (a.k.a. BRCC2, BROVCA2, FACD, FAD, FAD1, FANCD, FANCD1, GLM3, PNCA2, XRCC11)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

To construct p236BRCA2, the pcDNA3 vector was first modified by inserting a 236-bp fragment of the 5' untranslated region of BRCA2 between the KpnI and NotI sites. The assembled full-length BRCA2 cDNA was then inserted at the XhoI site of this plasmid. The 5' UTR of BRCA2 was obtained by RT-PCR using primers 5'-GGTACCGGTG GCGCGAGCTT CTGA-3' and 5'-GCGGCCGCAA CTACGATATT CCTCCAAT-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 236HSC WT (BRCA2) was a gift from Mien-Chie Hung (Addgene plasmid # 16246 ; http://n2t.net/addgene:16246 ; RRID:Addgene_16246)
  • For your References section:

    Inhibition of cancer cell growth by BRCA2. Wang SC, Shao R, Pao AY, Zhang S, Hung MC, Su LK. Cancer Res. 2002 Mar 1. 62(5):1311-4. PubMed 11888897