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pcDNA3.1(+)Flag-His-ATM wt
(Plasmid #31985)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31985 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    After transformation, grow for 2 days at 30 degrees. Ignore colonies that grow after 1 day. Let grow for a full 2 days.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATM wild-type
  • Alt name
    ATI
  • Alt name
    ATA
  • Alt name
    ATDC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9581
  • Mutation
    3' UTR of 400 nucl.
  • GenBank ID
    NP_000042.3/71902540
  • Entrez Gene
    ATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI-SalI Ligation (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)Flag-His-ATM wt was a gift from Michael Kastan (Addgene plasmid # 31985 ; http://n2t.net/addgene:31985 ; RRID:Addgene_31985)
  • For your References section:

    Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Canman CE, Lim DS, Cimprich KA, Taya Y, Tamai K, Sakaguchi K, Appella E, Kastan MB, Siliciano JD. Science. 1998 Sep 11;281(5383):1677-9. 10.1126/science.281.5383.1677 PubMed 9733515