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pcDNAFLAG-ATM-kd
(Plasmid #43908)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 43908 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNAFLAG
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ataxia telangiectasia mutated
  • Alt name
    ATM
  • Alt name
    HGNC:795
  • Alt name
    Entrez Gene: 472
  • Species
    H. sapiens (human)
  • Mutation
    kinase dead (D2870A and N2875K)
  • Entrez Gene
    ATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • His (N terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNAFLAG-ATM-kd was a gift from Stephen Elledge (Addgene plasmid # 43908 ; http://n2t.net/addgene:43908 ; RRID:Addgene_43908)
  • For your References section:

    Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. Cortez D, Wang Y, Qin J, Elledge SJ. Science. 1999 Nov 5. 286(5442):1162-6. 10.1126/science.286.5442.1162 PubMed 10550055